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Ammonia - Blood

Source Blood 
Mnemonic AMMON 
Specimen Requirements

RRL: 1 x 5 mL lavender EDTA tube.
0.2 mL plasma

VENOUS COLLECTION ONLY.

Minimum Blood Volume for Patients less than 45 kg (100 lb) Including Neonates and Pediatrics

Mix well by inverting tube 8 to 10 times.

MUST BE COLLECTED ON ICE.

PSC: Specimen not stable. Refer patient to FMC, ACH, or PLC. 
Specimen Handling

Deliver to lab immediately on ice.
Centrifuge without delay at 4ºC + 2ºC, aliquot, and place on ice. Plasma may be stored at 4ºC for up to 3 hours if necessary. Freeze for long term.

Microtainer Collections: Deliver to lab immediately on ice.  Centrifuge without delay in microcentrifuge at RT, do not aliquot, place on ice immediately.  Transport specimen on ice to testing lab ASAP.

Extra-regional: Immediately freeze plasma.  Ensure frozen specimen is packaged with appropriate packaging material so it remains frozen in transit.
DSC Accession will verify the specimen is received frozen and ensure it remains frozen for transport to FMC.
FMC Accession will verify the specimen is received frozen and ensure it is delivered frozen to Chemistry.
Chemistry will verify specimen is received frozen and rapidly thaw and process immediately.

If the specimen thaws before being received at FMC Chemistry it is unsuitable for analysis and must be rejected.  The specimen cannot be refrozen to continue in transit. 
Additional Information This test cannot be collected by Mobile Collection Services. 
Testing Location Chemistry-ACH, FMC & PLC 
Testing Frequency As required 
Alternate Name(s) NH4, NH3 
Reference Interval

12 - 47 mmol/L

Click here for critical values 


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