Chimerism Studies

Source	Blood or Bone Marrow
Mnemonic	DNAD PB (Blood - Donor) DNAR PB (Blood - Recipient) DNAD BM (Bone Marrow - Donor) DNAR BM (Bone Marrow - Recipient)
Specimen Requirements	Blood Adult: 1 x 8.5 mL yellow top ACD "A" tube Pediatric: 1 x 2.7 mL blue top sodium citrate tube. Lavender top EDTA tube is also acceptable; green top sodium heparin is NOT acceptable. Bone Marrow 0.5 mL in Flow Bone Marrow Medium (BMM); BMM available from Flow Cytometry. Stable for 24 hours.
Specimen Handling	DO NOT centrifuge or freeze. Send specimens to FMC Flow Cytometry within 24 hours of collection. DSC Accession: after 16:30, store at 4ºC.
Additional Information	Flow Cytometry will sort post-transplant cells and forward to Molecular Hematology for Chimerism Testing. Blood specimens must be accompanied by a Flow Cytometry requisition.
Testing Location	Molecular Hematology
Testing Frequency
Alternate Name(s)	Engraftment Studies, Flow Sort Chimerism
Reference Interval

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