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Hemostasis Study

Source Blood 
Mnemonic HS1 
Specimen Requirements

Adult: 4 x 5 mL blue top sodium citrate tube (4.5 mL draw)

Pediatric: 2 x short-draw (2.7 mL draw) blue top sodium citrate tube (minimum 1 short-draw tube, 2.7 mL)

Whole blood

When using a winged blood collection set (butterly needle) a discard tube must be used to remove the air in the tubing, if only a citrate tube is drawn or is the first tube drawn.

 
Specimen Handling

PSC and RRL: Send specimens, UNSPUN at room temperature to DSC Special Coagulation within 2 hours of collection.

Include copy of original requisition and Hemostasis Studies history form. If patient has received Desmopressin or Factor Concentrate, indicate medication, amount received, time of infusion/injection, IV or subcutaneous, patient weight.

DO NOT transport samples through pneumatic tube system.

Extra-regional: Centrifuge blue top tubes at 3500 rpm for 20 minutes, aliquot plasma into 5-6 plastic tubes. Freeze and transport frozen to DSC Special Coagulation.

 
Additional Information Note: Bleeding time and platelet aggregation studies must be booked with Special Coagulation.
Closure time is collected Monday to Friday 0700h - 1300h. No collection on statutory holidays.

If a Stat PT/PTT is required at ACH, draw one additional 1.8 mL draw plastic tube and indicate "STAT PT/PTT" on the requisition.
If unable to draw additional tube, submit all short-draw 2.7 mL tubes to Special Coagulation; indicate "STAT PT/PTT" on requisition. In this case the tests will be performed off-site and the turnaround time for results may be extended. 
Testing Location Special Coagulation 
Testing Frequency  
Alternate Name(s) Factor Assays (Coagulant, Factors II, V, VII, VIII, IX, X, XI, XII, XII screen), Inhibitor Screen/Assay, Mixing Study (Inhibitor Screen), Circulating Anticoagulants, Von Willebrand Workup (Von Willebrand Factor Antigen, Ristocetin Cofactor Activity) 
Reference Interval  


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