1 x 5 mL light green (mint) top PST tube. Gold top SST tube is also acceptable.
2.0 mL heparinized plasma or serum
Test includes: - Estriol, Unconjugated (E3) - Beta HCG, Quantitative - Blood - AFP
Testing should be performed between 15 3/7 to 16 3/7 weeks gestation, not earlier. Timing for ultrasound not crucial as calculation takes date into account.