Phosphate - Urine, Extra-regional

24 hour urine:
REFRIGERATE during collection.
Specimen may be acidified during or following collection, see Specimen Handling section for acidification instructions.

Random:
Follow acidification instructions in Specimen Handling.

Submit 5 mL aliquot of acidified 24 hour or random urine collection.

Acidify specimen in original collection container.
RECORD 24 hour volume and collection (start and end) dates and time in space provided on requisition.

24 Hour Collection:
< 5 years:         Collect in 5 mL of 6 mol/L HCl
5-10 years:       Collect in 10 mL of 6 mol/L HCl
10-15 years:     Collect in 15 mL of 6 mol/L HCl
15 years-Adult: Collect in 25 mL of 6 mol/L HCl

Random Collection:
Acidify with 6 mol/L HCl immediately after collection.
Shake bottle vigorously and LET SIT 15 minutes.
Mix and pour off 5 mL to aliquot tube.

Urine Volume	Volume of HCl to be added
100 mL	2000 µL
90 mL	1800 µL
80 mL	1600 µL
70 mL	1400 µL
60 mL	1200 µL
50 mL	1000 µL
40 mL	800 µL
30 mL	600 µL
20 mL	400 µL
10 mL	200 µL