Phosphate - Urine

Extra-regional sites: see Phosphate - Urine, Extra-regional

24 hour and Timed Collection:
REFRIGERATE during collection.
SUBMIT 5 mL aliquot of acidified 24 hour urine collection.
Specimen may be acidified during or following collection. See Specimen Handling and Additional Information sections for acidification instructions.

Random:
SUBMIT 5 mL aliquot of acidified random urine collection.
Specimen may be acidified during or following collection. See Specimen Handling and Additional Information sections for acidification instructions.

Acidify specimen in original collection container.

ADULT Collection Type	Weight of Sulfamic acid in pouch for ADULT Collections
24 hr. collections on patients > 4 yrs.	7 g pouch in each 4 liter container collection when acidifying during or after collection
Adult timed collections	Use 7 g pouch if > 700 mL or when acidifying during collection 0.5 g pouch for every 100 mL collected when acidifying after collection up to 700 mL
Adult random collections	0.5 g pouch for every 100 mL collected (orange capped urine container = 100 mL)

Collection Type for NEWBORN up to 4 YRS	Weight of Sulfamic acid in pouch for NEWBORN up to 4 YRS
24 hr. collections on newborn up to 4 yrs.	Use pre-acidification chart in Additional Information section
Random collections on newborn up to 4 yrs.	Refer patient to ACH

Aliquoting:
If acidified during urine collection: 
Mix well and pour off 5 mL of acidified urine to aliquot tube. 

If acidified following urine collection:
Add appropriate size of Sulfamic acid pouch, SHAKE bottle vigorously and LET SIT 15 minutes.
Mix and pour off 5 mL of acidified urine to aliquot tube.

RECORD 24 hour urine volume and collection (start and stop) dates and
times in space provided on requisition.

For Pre-acidification of Pediatric 24 hour Urines:

Age	Number of 0.5 g pouches to add when acidifying during collection
Up to 1 month	Not suitable for 0.5 g pouch Refer patient to ACH
1 up to 2 months	2
2 up to 4 months	3
4 up to 12 months	4
12 months up to 4 yrs.	5