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APL Transcript Analysis by PCR

Source Bone Marrow (preferred) or Blood
Mnemonic APL BM (Bone Marrow)
APL PB (Blood)
Specimen Requirements

Bone Marrow: 1 x 4 mL lavender top EDTA tube
NOTE: Bone Marrow is the preferred specimen type. 

Blood: 2 x 4 mL lavender top EDTA tubes.
Blood specimens must be collected before 13:30 Monday to Thursday (holidays excluded).

Specimen Handling Send specimens UNSPUN at room temperature to DSC Molecular Hematology within 4 hours of collection.

DSC Accession: after 16:30, store at 4°C.
Additional Information

Test can only be ordered by a Hematologist. 

Molecular investigations must be DEDICATED specimens to avoid sample contamination.

Peripheral blood specimens must be accompanied by a completed CLS Molecular Hematology requisition (REQ9053MH).

Bone marrow specimens must be accompanied by a completed Bone Marrow Pathology requisition (REQ9061BM).

Testing Location Molecular Hematology
Testing Frequency Monthly
Alternate Name(s) t(15:17) Translocation by PCR; AML(M3); APML
Reference Interval Not detected (Normal)