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Ammonia - Blood

Source Blood
Mnemonic AMMON
Specimen Requirements

Test cannot be collected by Patient Service Centres (PSC), Mobile Collection Service (MCS) or Urgent Care Centres.

RRL:
1 x 4 mL lavender EDTA tube.
0.2 mL plasma

Keep tubes cold (immerse in ice water) before collecting and immediately after collection.

VENOUS COLLECTION ONLY.

Minimum Whole Blood Volumes for Microcollections

Mix well by inverting tube 8 to 10 times.


PSC & Urgent Care Centres:
Specimen not stable. Refer patient to ACH, FMC, PLC, RGH or SHC.

Specimen Handling

Deliver to lab immediately on ice.
Centrifuge without delay at 4ºC + 2ºC, aliquot, and place on ice. Plasma may be stored at 4ºC for up to 1 hour if necessary. Freeze if unable to immediately analyze.

Microcollection Tubes (used for minimal volume venous collections):
Deliver to lab immediately on ice.  Centrifuge without delay in microcentrifuge at room temperature, do not aliquot, place on ice immediately.  Transport specimen on ice to testing lab as soon as possible.

Calgary Rural Labs:
Immediately freeze plasma. Ensure frozen specimen is packaged with appropriate packaging material so it remains frozen in transit.
Accession will verify the specimen is received frozen and ensure it is delivered frozen to Chemistry.
Chemistry will verify specimen is received frozen and rapidly thaw and process immediately.

Extra-regional:
Immediately freeze plasma.  Ensure frozen specimen is packaged with appropriate packaging material so it remains frozen in transit.
DSC Accession will verify the specimen is received frozen and ensure it remains frozen for transport to FMC.
FMC Accession will verify the specimen is received frozen and ensure it is delivered frozen to Chemistry.
Chemistry will verify specimen is received frozen and rapidly thaw and process immediately.

If the specimen thaws before being received in Chemistry it is unsuitable for analysis and must be rejected.  The specimen cannot be refrozen to continue in transit.
Additional Information
Testing Location Chemistry-ACH, FMC, PLC, RGH, SHC
Testing Frequency As required
Alternate Name(s) NH4, NH3
Reference Interval

12 - 47 umol/L

Click here for critical values