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Anti-Endomysial Antibodies

Source Blood
Mnemonic ANTIAEN  For Gastroenterology and referral specimens ONLY; refer to Celiac Disease Screen for all other patients
Specimen Requirements

1 x 3.5mL gold top SST tube

PSC only: 1 x 5 mL orange top RST tube is acceptable within one hour of lab closing.

Minimum Whole Blood Volumes for Micro-Collections

Specimen Handling
Additional Information Initiating a gluten-free diet prior to referral or prior to screening will interfere with the diagnostic tests for celiac disease. Please note that individuals may exhibit a wide range of responses to gluten challenge over varying periods of time.  Some patients only require a diet containing gluten (minimum of 3 g/day gluten or approximately 1-2 slices of bread/day) for at least 2 weeks to exhibit histological changes and at least 4 weeks for tTG IgA levels to increase.  Others may require a challenge for as long as two years prior to the development of intestinal injury or antibody response. Celiac disease serology during the challenge should be repeated every 4-6 months or immediately with the development of symptoms.

Click here to view a diagnostic algorithm.
Testing Location Immunochemistry
Testing Frequency Weekdays
Alternate Name(s) EMA
Reference Interval Anti-Endomysial Antibodies  - negative