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Chimerism Studies

Source Blood or Bone Marrow
Mnemonic

DNAD PB (Blood - Donor)
DNAR PB (Blood - Recipient)

DNAD BM (Bone Marrow - Donor)
DNAR BM (Bone Marrow - Recipient)

Specimen Requirements

Blood
Adult: 1 x 8.5 mL yellow top ACD "A" tube

Pediatric: 1 x 2.7 mL blue top sodium citrate tube.

Lavender top EDTA tube is also acceptable; green top sodium heparin is NOT acceptable.

Bone Marrow
0.5 mL in Flow Bone Marrow Medium (BMM); BMM available from Flow Cytometry. Stable for 24 hours.

Lavender top EDTA tube is also acceptable; green top sodium heparin tube is NOT acceptable.

Specimen Handling DO NOT centrifuge or freeze.

Send specimens to FMC Flow Cytometry within 24 hours of collection.

DSC Accession: after 16:30, store at 4 oC.
Additional Information Flow Cytometry will sort post-transplant cells and forward to Molecular Hematology for Chimerism Testing.

Blood specimens must be accompanied by a Flow Cytometry requisition.
Testing Location Molecular Hematology
Testing Frequency Bi-weekly
Alternate Name(s) Engraftment Studies, Flow Sort Chimerism
Reference Interval