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Cryofibrinogen

Source Blood
Mnemonic CRYOF
Specimen Requirements

Note to healthcare providers: test on patients under 14 years of age has restricted clinical indications and requires review and approval by Clinical Biochemist at ACH (403-955-2277) or CLS General Pathologist (403-860-1802). Test will not be collected without prior approval.

Collections performed at FMC, PLC, RGH and SHC.
Test cannot be collected by Mobile Collection Services (MCS), or at ACH, PSC or Urgent Care (ACHC, CCHC, SCHC, SMCHC) sites.
Redirect patient to FMC, PLC, RGH or SHC RRL Outpatient Lab (verify hours of operation).

Monday to Friday morning collections. Fasting specimens preferred.

1 x 6 mL lavender top EDTA tube AND 2 x 6 mL red top tube, NOT SST
Minimum amount after processing 2 mL each, serum and plasma.

RRL:
Use collection kits prepared by RRL Accession/Hematology.

CRL and Extra-Regional:
Follow instructions on Cryofibrinogen Collection Log HE4099.

Important: Use activated infant heel warmer to pre-warm tubes before collection and to maintain the specimen at 37ºC at all times. Use another heel warmer if necessary (temperature maintained for only 15 minutes once warmer is activated.)

Deliver tubes directly to FMC Hematology or to PLC, RGH or SHC Accession. DO NOT send tubes through the pneumatic tube system.

Specimen Handling

Hematology:

  • Place tubes immediately into 37ºC waterbath or 37ºC incubator.
  • Lavender top EDTA tube: Allow to sit for 1 hour at 37ºC (waterbath). Process as indicated below within 2 hours of collection.
  • Red top: Allow to clot for 1 hour at 37ºC (waterbath).
  • Centrifuge at room temperature for 15 minutes at 1700 rcf.  DO NOT use refrigerated or StatSpin centrifuge.
  • Separate into aliquot tubes labelled appropriately as “plasma” and “serum”.
  • Transport at room temperature to DSC.
Additional Information

Testing requires preapproval to be performed, contact Pathologist on call (403-860-1802).

If unable to collect two red top tubes, enter order note CRYOL (F9).

Testing Location Hematology-DSC
Testing Frequency Monday to Friday
Alternate Name(s)
Reference Interval Negative