Cryofibrinogen

Note to healthcare providers: test on patients under 14 years of age has restricted clinical indications and requires review and approval by CLS Medical & Scientific Staff.  Test will not be collected without prior approval.

Monday to Friday morning collections. Fasting specimens preferred.

1 x 6 mL lavender top EDTA tube AND 2 x 6 mL red top tube, NOT SST
Minimum amount after processing 2 mL each, serum and plasma.

PSC:
Redirect patient to FMC, PLC or RGH Outpatient Lab (verify hours of operation).
Not available at Alberta Children's Hospital.

RRL:
Use collection kits prepared by RRL Accession/Hematology.


Important: Use activated infant heel warmer to pre-warm tubes before collection and to maintain the specimen at 37ºC at all times. Use another heel warmer if necessary (temperature maintained for only 15 minutes once warmer is activated.)

Deliver tubes directly to FMC Hematology Department, PLC and RGH Accession Department.

Hematology:

• Place tubes immediately into 37ºC waterbath or 37ºC incubator.
• Lavender top EDTA tube: Allow to sit for 1 hour at 37ºC (waterbath). Process as indicated below within 2 hours of collection.
• Red top: Allow to clot for 1 hour at 37ºC (waterbath).
• Centrifuge at room temperature for 15 minutes at 1700 rcf.  DO NOT use refrigerated or StatSpin centrifuge.
• Separate into aliquot tubes labelled appropriately as “plasma” and “serum”.
• Transport at room temperature to DSC.

Testing requires preapproval to be performed, contact Hematology Division Head.

If unable to collect two red top tubes, enter order note CRYOL (F9).