Cryoglobulin

Note to healthcare providers: test on patients under 14 years of age has restricted clinical indications and requires review and approval by CLS Medical & Scientific Staff.  Test will not be collected without prior approval.

PSC:
Redirect patient to FMC, PLC, or RGH RRL Outpatient Lab (verify hours of operation).
Test is not collected at Alberta Children's Hospital or by Mobile Collection Service (MCS).


Patient should be fasting for 12 hours. Monday to Friday, morning collection preferred.

2 x 6 mL red top tubes, NOT SST
Minimum amount after processing 2 mL serum.

RRL:
Use collection kits prepared by RRL Accession/Hematology.

Important: Use activated infant heel warmer to pre-warm tubes before collection and to maintain blood specimen at 37°C at all times. Use another heel warmer if necessary (temperature maintained for only 15 minutes once warmer is activated).

Deliver tubes directly to FMC Hematology department, PLC and RGH Accession department.

Hematology:

• Place tubes immediately into 37°C waterbath or 37°C incubator. 
• Allow to clot for 1 hour at 37°C (waterbath).
• Centrifuge at room temperature for 15 minutes at 1700 rcf.  DO NOT use refrigerated or StatSpin centrifuge.
• Separate serum from both tubes into one labeled aliquot tube
• Transport at room temperature to DSC.