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Cryoglobulin

Source Blood
Mnemonic CRYOG
Specimen Requirements

PEDIATRIC COLLECTIONS:

Note to healthcare providers: test on patients under 14 years of age has restricted clinical indications and requires review and approval by Clinical Biochemist at ACH (403-955-2277) or CLS General Pathologist (403-860-1802). Test will not be collected without prior approval.

ADULT COLLECTIONS:

Collections performed at FMC, PLC, RGH and SHC.
Test cannot be collected by Mobile Collection Services (MCS) or at PSC or Urgent Care (ACHC, CCHC, SCHC, SMCHC) sites.
Redirect patient to FMC, PLC, RGH or SHC RRL Outpatient Lab (verify hours of operation).

Collections Monday to Friday 07:00 - 14:00 (Fasting preferred)


2 x 6 mL red top tubes, NOT SST
Minimum amount after processing 2.5 mL serum.

RRL:
Use collection kits prepared by RRL Accession/Hematology.

CRL and Extra-Regional:
Follow instructions on Cryoglobulin Collection Kit/Log Sheet HE4077.

Important:
Use activated infant heel warmer to pre-warm tubes before collection and to maintain blood specimen at 37°C at all times. Use another heel warmer if necessary (temperature maintained for only 15 minutes once warmer is activated).

Deliver tubes directly to FMC Hematology, or to PLC, RGH or SHC Accession. DO NOT send tubes through the pneumatic tube system.

Specimen Handling

Hematology:

  • Place tubes immediately into 37°C waterbath or 37°C incubator. 
  • Allow to clot for 1 hour at 37°C (waterbath).
  • Centrifuge at room temperature for 15 minutes at 1700 rcf.  DO NOT use refrigerated or StatSpin centrifuge.
  • Separate serum from both tubes into two labeled aliquot tubes
  • Transport at room temperature to DSC.

DSC Accession:
Deliver to Hematology. If after hours, store at room temperature.

Additional Information If unable to collect 2 red top tubes, enter order note CRYOL (F9). 
Testing Location Hematology-DSC
Testing Frequency Monday to Friday
Alternate Name(s)
Reference Interval Negative