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7-Dehydrocholesterol

Source Blood
Mnemonic BGLBL

BGL Test Name field: 7-Dehydrocholes
Specimen Requirements 1 x 4 mL dark green top sodium heparin tube. 1 x 4 mL dark green lithium heparin tube, NOT PST, and red top non-additive tube are also acceptable.

Minimum: 0.25 mL plasma or serum.

Requires a minimum four-hour fast, or just prior to next feed for newborns. 

Specimen Handling

Send a copy of the requisition with the specimen.

PSC:
Centrifuge, transfer plasma or serum to aliquot tube, write "NaHep", "LiHep" or "Red Top" on the aliquot tube, and transport on ice to DSC.

RRL:
Centrifuge, transfer plasma or serum to aliquot tube, write "NaHep", "LiHep" or "Red Top" on the aliquot tube, and transport on ice to ACH.

Extra-regional:
Centrifuge, transfer plasma or serum to aliquot tube, write "NaHep", "LiHep" or "Red Top" on the aliquot tube, and freeze prior to transport to DSC.

DSC:
Freeze plasma or serum upon receipt in lab, ensure frozen specimens do not thaw.
  

Additional Information This test is for the investigation of Smith-Lemli-Opitz syndrome, and the level of 7-dehydrocholesterol is reported along with the cholesterol level.
Testing Location ACH Biochemical Genetics Lab
Testing Frequency Monthly; must notify ACH Biochemical Genetics Lab for priority turn-around
Alternate Name(s) (3B)-cholesta-5,7-dien-3-ol; 7DHC
Reference Interval Provided on test report.