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Donath-Landsteiner Test

Source Blood
Mnemonic DON LAN
Specimen Requirements 2 x 6 mL red top tube
Specimen Handling Keep specimen at 37ºC until separated.
Allow to clot for 1 hour at 37ºC. 
Centrifuge specimen at 1700RCF for 15 minutes.
Immediately remove from centrifuge and separate serum into labelled tubes. At least 3 mL of serum is required.
Deliver serum aliquot to FMC TM.
Additional Information Physicians should prearrange collection and testing through Transfusion Medicine FMC at 403-944-1367.
Testing Location Transfusion Medicine-FMC
Testing Frequency As required
Alternate Name(s)
Reference Interval