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Fecal Fat, 72 Hour

Source Stool
Mnemonic FECAL
Specimen Requirements

72 hour stool collection in pre-weighed container available through Calgary Laboratory Services Patient Service Centres or hospital Accession departments (specimens received in any other container are subject to possible specimen rejection). If a specimen of <72 hours is received, please advise the patient to continue the collection for the full 72 hours if possible.

Record collection (start and end) dates and times on requisition.

DO NOT perform during Barium Swallow procedure.

Supply patient with collection instruction sheet (#CH3022) and diet information sheet (#CH3012). 

Extra-regional sites can obtain collection containers by emailing Shipping/ReceivingDept@cls.ab.ca.

PSC & RRL:
Send all specimens to ACH Chemistry.

Specimen Handling Copy of requisition must be sent with specimen.
Additional Information

The chemical method for fecal fat has been replaced with an assessment of 72-hour specimen weight, and a qualitative test for fat globules (STFAT) as described in Korostensky et. al. The Journal of applied Laboratory Medicine. June 2018.

Specimen weight will be compared to age-specific cut-points with either 45% (7-150 years of age) or 55% (<7 years of age) sensitivity and 80% specificity for abnormal fat excretion (7-150 years: >20.9 mmol/day; <7 years: >6.9 mmol/day). Specimen weight greater than cut-points will be reported as 'HIGH' in a comment.

The weight assessment should be interpreted in light of results from the test for fat globules (STFAT).

Any specimen collections <72 hours collection are NOT acceptable for weight assessment. Specimens collected for <72 hours will only be tested for STFAT.

Note: Additional "random" tests will not be performed on 72 hour collections unless approval has been obtained by CLS Scientific and Medical staff.

Testing Location Chemistry-ACH
Testing Frequency

Daily

Alternate Name(s) Fecal Fat, Quantitative; Fat, Quantitative
Reference Interval

72-hour specimen weight assessment

Less than 7 years

<196 grams

Note: Specimen weight greater than or equal to 196 grams has 55% sensitivity and 80% specificity for fecal fat excretion > 6.9 mmol/day

7-150 years

<947 grams

Note: Specimen weight greater than or equal to 947 grams has 45% sensitivity and 80% specificity for fecal fat excretion >20.9 mmol/day

Click here for link to Fat Globules, Random - Stool  (STFAT)