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HLA B-27 by Flow Cytometry

Source Blood
Mnemonic HLA B27
Specimen Requirements

Calgary Zone Collections (including CRL sites)
Patients of all ages: 1 x 4 mL dark green top sodium heparin (ACD-A is also acceptable. Lithium heparin is NOT acceptable.)

Minimum volume: 0.5 mL


Extra-Regional Collections
Pediatric: 1 x 1.8 mL blue top sodium citrate

Adult: 1 x 8.5 mL yellow top ACD-A

Minimum volume: 0.5 mL

Specimen Handling

Specimens submitted to CLS from within Alberta: Mix well. DO NOT refrigerate, centrifuge or freeze. Send on regular courier run. Sample stable for 5 days at room temperature.

Specimens submitted to CLS from outside Alberta: DO NOT centrifuge or freeze samples. Ship at 4oC, using an overnight courier. Please follow Flow Cytometry shipping and handling instructions when sending to CLS. Sample stable for 5 days at room temperature.

Additional Information

Specimens submitted to CLS from outside Alberta: Use FC3200EP Flow Cytometry Out of Province Requisition.
For pricing and billing, refer to Flow Cytometry Billing and Pricing.

Testing Location Flow Cytometry
Testing Frequency

Monday - Saturday

Alternate Name(s) B-27 Antigen, B27
Reference Interval Provided on report.