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Hemoglobin A1c

Source Blood
Mnemonic

HBA1C

If "Endocrinology HBA1C" or "ENDOA1C" is ordered, see Endocrinology Hemoglobin A1c.

Specimen Requirements

1 x 4 mL lavender top EDTA tube

Minimum Whole Blood Volumes for Microcollections

AHC Outpatient Lab only: Capillary tube collections are available for patients from the ACH Endocrine and Diabetes Clinic only. Refer to Cobas c513 HbA1c Capillary Collection System Instructions for ACH CH3190.

Whole blood

Specimen Handling Mix well by inverting tube 8 to 10 times.

DO NOT centrifuge.
Additional Information

HbA1c results are affected by conditions that affect RBC lifespan (e.g. higher in iron deficiency, lower in chronic blood loss).  Glucose, Fasting or Fructosamine may be more appropriate tests in these cases.

Effective October 27, 2015, new provincial HbA1c test utilization criteria have been implemented in CLS urban and rural laboratories to align with the recommendation by the 2013 Canadian Diabetes Association.  Only one HbA1c request will be performed within a 90 day period.  For female patients 15-45 years inclusive, only one HbA1c request will be performed within a 30 day period (this is intended for those patients who are pregnant and require treatment). Only requests meeting these ordering frequencies will be collected and processed.  For further information on HbA1c testing please refer to Canadian Journal of Diabetes 37 (2013) S35-39 and the AHS Lab Bulletin for physicians.

The Hemoglobin A1c method used is DCCT/NGSP standardized.

CLS and CRL staff:
Data enter the test as requested; PathNet will determine if the test will be collected and will print appropriate labels. DO NOT ask the patient's age, or if they are pregnant. If PathNet determines the test will not be collected, provide patient with a Hemoglobin A1c Patient Information Letter.

Specimens will be routed to DSC Hematology for sorting. DSC Hematology will forward specimens to DSC Chemistry for testing. Use false bottom tubes if aliquotting is required.

Testing Location Chemistry-DSC
Testing Frequency Weekdays
Alternate Name(s) Glycohemoglobin
Reference Interval < 6.5%