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Hemostasis Study

Source Blood

 
Mnemonic HS1

 
Specimen Requirements

Test cannot be collect by Mobile Collection Service (MCS).

Adult:
4 x 4.5 mL blue top sodium citrate tube

Pediatric:
2 x 2.7 mL draw short-draw blue top sodium citrate tube (minimum 1 x 2.7 mL short-draw tube)

Whole blood

When using a winged blood collection set (butterly needle) a discard tube must be used to remove the air in the tubing, if only a citrate tube is drawn or is the first tube drawn.

If "pre-" or "post-infusion" is indicated on the requisition, collect the following tubes.  Contact Special Coagulation at 403-770-3598 for confirmation of minimum volumes if unable to draw recommended volume: 
Adult pre-infusion: 2 x 4.5 mL blue top sodium citrate tube
Adult post-infusion: 1 x 4.5 mL blue top sodium citrate tube
Pediatric pre-infusion: 2 x 2.7 mL draw short-draw blue top sodium citrate tube (minimum 1 x 2.7 mL short-draw tube)
Pediatric post-infusion: 1 x 2.7 mL draw short-draw blue top sodium citrate tube

 

Specimen Handling

PSC and RRL:
Send specimens, UNSPUN at room temperature to DSC Special Coagulation within 2 hours of collection.

Note: Always send copy of original requisition and Hemostasis Studies history form. If patient has received Desmopressin or Factor Concentrate, indicate medication, amount received, time of infusion/injection, IV or subcutaneous, patient weight.

DO NOT transport samples through pneumatic tube system.


Extra-regional:
Centrifuge blue top tubes at 3500 rpm for 20 minutes, aliquot plasma into 5-6 plastic tubes. Freeze and transport frozen to DSC Special Coagulation.
 
 

Additional Information Note: Bleeding time and platelet aggregation studies must be booked with Special Coagulation.
Closure time is collected Monday to Friday 07:00 - 13:00. No collection on statutory holidays.

If a Stat PT/PTT is required at ACH, draw one additional 1.8 mL draw plastic tube and indicate "STAT PT/PTT" on the requisition.
If unable to draw additional tube, submit all short-draw 2.7 mL tubes to Special Coagulation; indicate "STAT PT/PTT" on requisition. In this case the tests will be performed off-site and the turnaround time for results may be extended.

  
Testing Location Special Coagulation
Testing Frequency
Alternate Name(s) Factor Assays (Coagulant, Factors II, V, VII, VIII, IX, X, XI, XII, XII screen), Inhibitor Screen/Assay, Mixing Study (Inhibitor Screen), Circulating Anticoagulants, Von Willebrand Workup (Von Willebrand Factor Antigen, Ristocetin Cofactor Activity)

 
Reference Interval