Employee Login  |   Subscribe  |   Careers  |   Appointments
List All Test Names Beginning With:
A B C D E F G H I J K L M N O P Q R S T U V W X Y Z

Hyper IgM Syndrome Screen

Source Blood
Mnemonic LINK
Specimen Requirements

Pediatric: 1 x 4 mL dark green top sodium heparin tube

Adult: 2 x 4 dark green top sodium heparin tubes

Minimum volume: 1.0 mL

Not accepted: EDTA, sodium citrate, ACD-A

Specimen Handling

Specimens submitted to CLS from within Alberta: Mix well. DO NOT refrigerate, centrifuge or freeze. Deliver immediately to Flow Cytometry.

Specimens submitted to CLS from outside Alberta: DO NOT refrigerate, centrifuge or freeze samples. Ship at room temperature, using an overnight courier. Please follow Flow Cytometry Shipping and Handling instructions when sending to CLS.

Additional Information

Specimens submitted to CLS from outside Alberta: Use FC3200EP Flow Cytometry Out of Province Requisition.
For pricing and billing, refer to Flow Cytometry Billing and Pricing.

Two assays are used to screen patients for Hyper IgM Syndrome (HIM). CD40 deficiency (HIM Type 3) results from a functional biallelic mutation of the CD40 gene (20q12q13.2) resulting in an autosomal recessive inheritance pattern. CD40 expression is assessed on peripheral blood B cells by flow Cytometry and is reported as present/absent. CD40L deficiency (HIM Type 1) results from a CD154 gene mutation on the X chromosome. Peripheral blood T cells are activated with PMA/Ionomycin and assessed for CD154 expression on the surface of CD3+4+8- cells. 

Testing Location Flow Cytometry
Testing Frequency Monday - Friday
Alternate Name(s)

CD40, CD40L, HIM, LINK, X-Linked Hyper IgM Syndrome Screen

Reference Interval Provided on report.