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Invariant NK Cells

Source Blood
Mnemonic INKT
Specimen Requirements

Pediatric: 1 x 4 mL dark green top sodium heparin tube

Adult: 1 x 4 mL dark green top sodium heparin tube

Minimum volume: 0.5 mL

Also accepted: EDTA:
Not accepted: ACD-A, sodium citrate, lithium heparin

Specimen Handling

Specimens submitted to CLS from within Alberta: Mix well. DO NOT centrifuge or freeze. Deliver immediately to Flow Cytometry.

Specimens submitted to CLS from outside Alberta: DO NOT centrifuge or freeze samples. Ship at room temperature or 4oC, using an overnight courier. Please follow Flow Cytometry Shipping and Handling instructions when sending to CLS.

Additional Information

Specimens submitted to CLS from within Alberta: Samples should be tested within 48 hours of collection.

Specimens submitted to CLS from outside Alberta: Use FC3200EP Flow Cytometry Out of Province Requisition.
For pricing and billing, refer to Flow Cytometry Billing and Pricing.

iNKT deficiency can be seen in XLP1 (SH2D1A gene mutation), Omenn syndrome, CVID and Hyper IgE syndrome. Peripheral blood iNKT cell enumeration is performed by flow Cytometry based on co-expression of CD3, TCRvα24 and TCRvβ11. The percentage and absolute iNKT count are reported.

Testing Location Flow Cytometry
Testing Frequency Monday - Saturday
Alternate Name(s) INKT
Reference Interval Provided on report.