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Lactose Tolerance

Source Blood
Mnemonic LTT
Specimen Requirements

By appointment only.
Community patients: contact CLS Patient Appointment Line at 403-777-5136 to book an appointment at any Patient Service Centre. Test cannot be collected by Mobile Collection Service (MCS).
Hospital patients: nursing unit to book appointment with on-site lab. 

Pediatric patients under 5 years of age:
Due to possible difficulty administering the drink, ordering physician must obtain approval by CLS Medical and Scientific Staff.  Contact Laboratory Information Center at 403-770-3600 for Pathologist-on-call information. An alternative non-invasive test is the Hydrogen Breath Test for Lactose Intolerance.


1 x 3 mL or 4.5 mL light green (mint) top PST tube for each timed specimen.  Gold top SST tube also acceptable.

0.2 mL heparinized plasma or serum

Minimum Whole Blood Volumes for Micro-Collections using Microtainers

Requires a fast of at least 8 hours.

Blood collections are performed at the following intervals: fasting, 30 minutes, 60 minutes, and 120 minutes.

Specimen Handling Procedure takes 2 hours.                            
Additional Information

Patient instruction sheet (form #CH3002) may be given to patient.

Order drink at least 2 days in advance.

  • Sloane Square & Marlborough PSC, & RRL: order from CLS Purchasing.
  • Other PSC sites: order from Sloane Square or Marlborough PSC.
  • Extra-regional: order from DSC Accession at 403-770-3245


All PSC & extra-regional:
If patient weighs less than 23 kg (50 lb), order drink from DSC Accession at 403-770-3245 and state patient's weight.

The drink provided for Lactose Tolerance test includes the following ingredients:

  • Lactose monohydrate
  • Natural orange flavouring
  • No artificial flavouring or colouring
Testing Location Chemistry-ACH, DSC, FMC, PLC & RGH
Testing Frequency Weekdays
Alternate Name(s)
Reference Interval A deficiency of lactase, or a malabsorption problem, is shown by a flat or almost flat curve, with the rise in glucose over the fasting level being less than 1.1 mmol/L