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Lymphocyte Activation Markers

Source Blood
Mnemonic LAM
Specimen Requirements

Pediatric: 1 x 4 mL dark green top sodium heparin

Adult: 2 x 4 mL dark green top sodium heparin

Minimum volume: 2.0 mL

Not accepted: EDTA, sodium citrate, ACD-A, lithium heparin

Specimen Handling

Specimens submitted to CLS from within Alberta. Mix well. DO NOT centrifuge or freeze. Deliver immediately to Flow Cytometry.

Specimens submitted to CLS from outside Alberta: DO NOT centrifuge or freeze samples. Ship at room temperature or 4oC, using an overnight courier. Please follow Flow Cytometry Shipping and Handling instructions when sending to CLS.

Additional Information

Specimens submitted to CLS from outside Alberta: Use FC3200EP Flow Cytometry Out of Province Requisition.
For pricing and billing, refer to Flow Cytometry Billing and Pricing.

CD3+4+ cells are assessed for surface expression of CD154, CD69, HLA-DR, CD143, CD25, CD71, and CD95 pre and post PMA/Ionomycin stimulation. Panel 2: CD8+ T cells are assessed for expression of CD45RO and HLA-DR primarily for use in investigation of Omenn's Syndrome of HLH. Antigen density of CD5 on CD3+8+ cells is also reported for HLH investigations.

Testing Location Flow Cytometry
Testing Frequency Monday - Thursday
Alternate Name(s) LAM
Reference Interval Provided on report.