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Neutrophil Function, Oxidative Burst

Source Blood
Mnemonic NFUN
Specimen Requirements

Pediatric: 1 x 1.8 mL blue top sodium citrate

Adult: 1 x 8.5 mL yellow top ACD-A

Minimum volume: 0.5 mL

Also accepted: EDTA, sodium heparin

Specimen Handling

Specimens submitted to CLS from within Alberta: Mix well. DO NOT refrigerate, centrifuge or freeze. Deliver immediately to Flow Cytometry

Specimens submitted to CLS from outside Alberta: DO NOT refrigerate, centrifuge or freeze samples. Ship at room temperature, using an overnight courier.  Please follow Flow Cytometry Shipping and Handling instructions when sending to CLS.

Additional Information

Specimens submitted to CLS from within Alberta: Samples should be tested within 24 hours of collection.

Specimens submitted to CLS from outside Alberta: Use FC3200EP Flow Cytometry Out of Province Requisition.
For pricing and billing, refer to Flow Cytometry Billing and Pricing.

Neutrophils are stained with the cell permeant, non-fluorescent dye dihydrorhodamine 123 and then stimulated with PMA. Degranulation releases molecules including peroxide, oxygen radicals, etc. which then oxidize the substrate dye into a brightly fluorescent product which is quantitated by flow cytometry. The percentage and fluorescent intensity of neutrophils demonstrating bright fluorescence post-PMA stimulation is reported.

Testing Location Flow Cytometry
Testing Frequency Monday - Friday
Alternate Name(s) DHR, Oxidative Burst, Respiratory Burst
Reference Interval Provided on report.