List All Test Names Beginning With:
|A B C D E F G H I J K L M N O P Q R S T U V W X Y Z|
Pediatric: 1 x 1.8 mL blue top sodium citrate
Adult: 1 x 8.5 mL yellow top ACD-A
Minimum volume: 0.5 mL
Also accepted: EDTA, sodium heparin
Specimens submitted to CLS from within Alberta: Mix well. DO NOT refrigerate, centrifuge or freeze. Deliver immediately to Flow Cytometry
Specimens submitted to CLS from outside Alberta: DO NOT refrigerate, centrifuge or freeze samples. Ship at room temperature, using an overnight courier. Please follow Flow Cytometry Shipping and Handling instructions when sending to CLS.
Specimens submitted to CLS from within Alberta: Samples should be tested within 24 hours of collection.
Specimens submitted to CLS from outside Alberta: Use FC3200EP Flow Cytometry Out of Province Requisition.
Neutrophils are stained with the cell permeant, non-fluorescent dye dihydrorhodamine 123 and then stimulated with PMA. Degranulation releases molecules including peroxide, oxygen radicals, etc. which then oxidize the substrate dye into a brightly fluorescent product which is quantitated by flow cytometry. The percentage and fluorescent intensity of neutrophils demonstrating bright fluorescence post-PMA stimulation is reported.
|Testing Location||Flow Cytometry
|Testing Frequency||Monday - Friday|
|Alternate Name(s)||DHR, Oxidative Burst, Respiratory Burst|
|Reference Interval||Provided on report.|