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Perforin and Granzyme B Protein Expression

Source Blood
Mnemonic HLH
Specimen Requirements

Pediatric: 1 x 1.8 mL blue top sodium citrate

Adult: 1 x 8.5 mL yellow top ACD-A

Minimum volume: 1.0 mL

Also accepted: EDTA
Not accepted: sodium heparin

Specimen Handling

Specimens submitted to CLS from within Alberta: Mix well. DO NOT refrigerate, centrifuge or freeze. Deliver immediately to Flow Cytometry.

Specimens submitted to CLS from outside Alberta: DO NOT refrigerate, centrifuge or freeze samples. Ship at room temperature, using an overnight courier. Please follow Flow Cytometry Shipping and Handling instructions when sending to CLS.

Additional Information

Specimens submitted to CLS from within Alberta: Samples should be tested within 24 hours of collection.

Specimens submitted to CLS from outside Alberta: Use FC3200EP Flow Cytometry Out of Province Requisition.
For pricing and billing, refer to Flow Cytometry Billing and Pricing.

As a screen for familial hemophagocytic lymphohistiocytosis (HLH), NK cells are assessed for the presence of the cytoplasmic molecules perforin and granzyme B. The percentage of CD3-CD16/56+ NK cells expressing perforin and granzyme B are reported.

Testing Location Flow Cytometry
Testing Frequency Monday - Saturday
Alternate Name(s) Hemophagocytic Lymphohystiocytosis, HLH Screen
Reference Interval Provided on report.