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Philadelphia Chromosome Transcript Analysis by Nested PCR

Source Blood or Bone Marrow
Mnemonic PHLR PB (Blood)
PHLR BM (Bone Marrow)
Specimen Requirements

Blood: 2 x 4 mL lavender top EDTA tube
Blood specimens must be collected before 13:30 Monday to Thursday (holidays excluded). For patients arriving after 13:30, please contact Molecular Hematology at 403-770-3699, option 2, prior to collection.

Rural sites: Please contact Molecular Hematology at 403-770-3699, option 2, prior to collection.

Bone Marrow: 1 x 4 mL lavender top EDTA tube

Specimen Handling

All sites: Send specimens UNSPUN at room temperature to DSC Molecular Hematology within 4 hours of collection.

Rural sites: DO NOT centrifuge. If delay in transport, store at 4ºC. Specimens must be received by Molecular Hematology no later than 15:00 the day following collection, receipt Monday to Friday only, statutory holidays excluded.

DSC Accession: After 16:30, store at 4oC.

Additional Information

Molecular investigations must be DEDICATED specimens to avoid sample contamination.

Peripheral blood specimens must be accompanied by a completed CLS Molecular Hematology requisition (REQ9053MH).

Bone marrow specimens must be accompanied by a completed Bone Marrow Pathology requisition (REQ9061BM).

Testing Location Molecular Hematology
Testing Frequency Monthly
Alternate Name(s) Philadelphia Chromosome (for exclusion); t(9:22) Translocation by Nested PCR; bcr/abl
Reference Interval Not detected (Normal)