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QuantiFERON TB Gold IT

Referred out to Alberta Provincial Laboratory for Public Health (ProvLab)

Consult the ProvLab Guide to Services for test requirements and complete the appropriate ProvLab requisition available at: http://www.albertahealthservices.ca/lab/Page3317.aspx.

Source Blood
Mnemonic QF TB
Specimen Requirements

QuantiFERON can only be ordered by the SAC Clinic and the TB Clinic, and can ONLY be collected at Sheldon Chumir and Sunridge PSCs (adult or pediatric patients) and ACH (pediatric patients ONLY). Test can also be collected on hospital inpatients with MVOC (Microbiologist or Virologist On Call) approval.

Test cannot be collected by Mobile Collection Services (MCS).

QuantiFERON kits are distributed by Provincial Laboratory.

Use a winged infusion set (butterfly) and syringe to collect blood.
Using a transfer device, transfer 1.0 mL blood to each of the 4 tubes (grey, green, yellow and purple) in the kit provided by the patient. Ensure tubes are completely filled to the 1 mL fill line. Do not overfill or under-fill tubes.


Immediately after filling the tubes, shake the tubes 10 times just firmly enough to ensure the entire inner surface of the tube has been coated with blood. Over-energetic shaking may cause gel disruption and could lead to aberrant results.

CLS staff:
 - Apply the "QF NIL" label to the grey top tube
 - Apply the "QF TBAg1" label to the green top tube
 - Apply the "QF TBAg2" label to the yellow top tube
 - Apply the "QF Mitogen" label to the purple top tube

The signature of collector and date and time of collection MUST be written on requisition, or sample may be rejected.

Specimen Handling Tubes should be at room temperature. Specimens must arrive at Provincial Lab within 16 hours of collection.

Sunridge and Sheldon Chumir PSC:
Before 14:00: do not centrifuge, refrigerate or freeze. Transport to DSC.
After 14:00: hold specimens until 18:00 (Sunridge) or 16:30 (Sheldon Chumir). Do not centrifuge, refrigerate or freeze. Transport specimens directly to Provincial Lab (attach Prov Lab tag to transport box).

RRL Accession:
Do not centrifuge, refrigerate or freeze. Transport directly to Provincial Lab.

DSC Accession:
Do not centrifuge, refrigerate or freeze. Transport to Provincial Lab in next courier run.

Additional Information

Testing Location Referred out to ProvLab
Testing Frequency
Alternate Name(s) QuantiFERON
Reference Interval