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Molecular Diagnostics - Molecular Hematology

The Molecular Hematology Laboratory located at CLS’ Diagnostic and Scientific Centre in the University Research Park provides advanced molecular and biochemical testing in the areas of molecular analysis for diagnosis of alpha thalassemia and inherited disorders of coagulation, and the diagnosis and monitoring of patients with hematologic malignancies.

The laboratory provides advanced molecular analysis (e.g. Polymerase Chain Reaction (PCR)) for diagnosis, prognosis, and monitoring of patients with myeloproliferative neoplasms and leukemias. This includes monitoring patients for treatment response after tyrosine kinase inhibitors or chemotherapy, and disease recurrence and graft survival after bone marrow or stem cell transplantation.

As a regional centre for testing in hemostasis, the laboratory offers DNA molecular diagnostics (gene testing) for inherited disorders such as hemophilia A and B, von Willebrand disease, and for inherited risk factors in thrombosis. The laboratory works closely with the Special Coagulation laboratory at CLS to provide integrated reporting and interpretation of functional, antigenic and molecular data.

Also, as a provincial centre for alpha thalassemia mutation analysis, Molecular Hematology supports Special Hematology in the investigation of hemoglobinopathies.

Technologies employed include: western blotting for Von Willebrand factor (vWF) protein multimer analysis, qualitative and quantitative PCR mutation detection for diagnosis and prognosis in acute leukemia, kinetic (real time PCR) for minimal residual disease determination, short tandem repeat (STR) and capillary electrophoresis for identity testing and hematopoietic cell chimerism analysis on whole blood, bone marrow and flow separated cells, modified inverse PCR (iPCR) for factor VIII gene inversion analysis in hemophilia A, and whole blood, hemi-nested allele specific PCR for inherited risk factors of thrombosis (Prothrombin, and Factor V Leiden). GAP PCR is utilized for the detection of seven common deletional variants associated with alpha thalassemia.

Our clients include: the Bleeding Disorders clinics in Calgary and in Edmonton, the Bone Marrow Transplant (BMT) program at the Tom Baker Cancer Centre (TBCC), and Hematology/Oncology and Thrombosis/Hemostasis programs including; extra-regional clients in Red Deer, Lethbridge, Medicine Hat, Edmonton, and South-Eastern British Columbia. 

The laboratory participates in clinical research, method development, and medical education through accredited and advanced residency training programs (Pathology, Hematopathology, Pediatric Oncology and Clinical Hematology)


Advanced testing requires a core of highly trained technical staff (all with undergraduate degrees and/or Medical Laboratory Technologist (MLT) diplomas), proficient in advanced molecular technologies, complex methodology, and with trouble-shooting, QA/QC, and method development skill sets. Laboratory currently employs 7.2 FTE equivalent technical staff and one Clinical Scientist.


Laboratory Leader:

Gary D. Sinclair, PhD, AACLDS
Tel:  (403) 770-3594
Fax:  (403) 770-3738


Tel:  (403) 770-3699
Fax:  (403) 770-3786

Postal Address:

Molecular Hematology
Calgary Laboratory Services
9, 3535 Research Road NW
Calgary, AB  T2L 2K8