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Blood Collection Supplies


  • Use a new tourniquet for each patient.   
  • Tourniquets should not be left on the patient for more than 1 minute.

Multi-sample Needles

  • Sterile, single use, double ended multiple sample needles, with safety device. These needles are used with the Vacutainer system for the majority of collections.
  • The higher the gauge number of a needle, the smaller the bore (opening) it has: available in 21G (green) and 22G (black).
  • Routinely 21G needles are used for faster tube filling rates, and 22G needles are used on smaller veins.

Butterfly Needles

  • Winged infusion sets with a safety device.
  • Used for blood culture collections.
  • Used on difficult veins such as hand or foot veins.
  • May also be used for syringe collection.

Needles (Syringe)

  • Sterile, single use needles used with a syringe for blood collection. 
  • Used for smaller veins that may collapse with Vacutainer collection.   
  • Samples are transferred from the syringe into blood collection tubes with the use of a Transfer Device.

Vacutainer Holders

  • Single-use holders are used for Vacutainer system collections.
  • Larger size is available for blood culture collections.


  • 70% isopropyl alcohol is used for routine blood collection. 
  • PVP Iodine is used for blood culture collections in combination with 70% isopropyl alcohol.
  • Chlorhexidine gluconate may be used at some sites instead of isopropyl alcohol for routine blood collection.

Collection Supplies: Safety and Notes

  • Standard precautions must be observed for all blood collections.
  • Re-capping of needles is against the law in Alberta.
  • Vacutainer multisample needle collection is preferred. Use of syringes with needles and butterfly sets is discouraged due to higher incidence of needle-stick injuries.
  • CLS does not accept specimens in syringes with needles still attached.