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Blood Collection Supplies



  • Tourniquets should be placed 8 - 10 cm above the puncture site (exception: pediatric patients and smaller arms).
  • Tourniquets should not be left on the patient for more than 1 minute.


Multi-sample Needles


  • Sterile, single use, double ended multiple sample needles, Eclipse safety guard, packaged in a plastic case. These needles are used with the Vacutainer system for the majority of collections.
  • The higher the gauge number of a needle, the smaller the bore (opening) it has: available in 21G (green) and 22G (black).
  • Routinely 21G needles are used for faster tube filling rates, and 22G needles are used on smaller veins.


Butterfly Needles


  • Winged infusion sets with a safety device.
  • Used for blood culture collections.
  • Used on difficult veins such as hand or foot veins.
  • May also be used for syringe collection.


Needles (Syringe)


  • Sterile, single use needles used with a syringe for blood collection. 
  • Used for smaller veins that may collapse with Vacutainer collection.   
  • Samples are transferred from the syringe into blood collection tubes.


Vacutainer Holders


  • Single-use holders are used for Vacutainer system collections.
  • Larger sizes available for blood culture collections.




  • 70% isopropyl alcohol is used for routine blood collections. 
  • PVP Iodine is used for blood culture collections in combination with 70% isopropyl alcohol.


Collection Supplies: Safety and Notes

  • Standard precautions must be observed for all blood collections.
  • Re-capping of needles is against the law in Alberta.
  • Vacutainer multisample needle collection is preferred. Use of syringes with needles and butterfly sets is discouraged due to higher incidence of needle-stick injuries.
  • CLS does not accept specimens in syringes with needles still attached.
  • Laboratory Assistants are not authorized under the Criminal Code of Canada to collect specimens for law enforcement.
  • CLS does not collect, handle or process blood gas specimens.