B Cell Subsets

Source	Blood
Mnemonic	BSUBSETS (Note: CBC/DIFF required if not already collected same day)
Specimen Requirements	1 x 4 mL dark green top sodium heparin tube. NOT PST. Minimum volume: 1 mL Also accepted: EDTA, sodium citrate, ACD-A
Specimen Handling	Mix well. DO NOT centrifuge or freeze. Specimens submitted to CLS from outside Alberta: CBC/DIFF required: fax CBC/DIFF results to 403-270-4135. DO NOT centrifuge or freeze specimens. Follow Flow Cytometry Shipping and Handling instructions when sending to CLS.
Additional Information	The following B cell subsets are enumerated: memory, naïve, memory non-class switched, memory class switched, memory IgM+, transitional, activated CD21^low, immature CD21^low, plasmablasts.
Testing Location	Flow Cytometry
Testing Frequency	Monday - Friday
Alternate Name(s)
Reference Interval	Shown on report.