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Bilirubin - Blood

Source Blood
Mnemonic
 Patients less than 15 days  
      Total Bilirubin  NBILT
      Total and Direct Bilirubin  NBILTD
      "Bilirubin" or "Bili" written on requisition   NBILT
   
 Patients greater than 15 days  
      Total Bilirubin  BILT
      Total and Direct Bilirubin  BILTD
      "Bilirubin" or "Bili" written on requisition  BILT
      Direct Bilirubin (only as add-on to BILT)  BILD

Note: For patients 15 days to 5 months less one day, PathNet will automatically add a Direct Bilirubin to all Total Bilirubin orders.
Specimen Requirements 1 x 3 mL or 4.5 mL light green (mint) top PST tube.  Gold top SST tube also acceptable.

0.2 mL plasma or serum

Minimum Whole Blood Volumes for Microcollections

Protect infant collections from light by using gold top amber coloured microcollection tube or green top tube with amber sleeve cover. Specimens not protected from light will be rejected.
Specimen Handling
Additional Information

If Indirect Bilirubin is required, make a note on the requisition.
Indirect Bilirubin is a calculated result of Total Bilirubin and Direct (Conjugated) Bilirubin.

Chemistry-ACH, DSC, FMC, PLC, RGH, SHC, ACHC, CCHC, SCHC, SMCHC will test Total and/or Direct Bilirubin

Rural CRL will test Total and Neonatal Bilirubin; Direct Bilirubin is only available at the following rural locations: BMSH, CMGH, DDHS, HRH, OGH, SDHS.

Indocyanine green interferes with Total and Direct Bilirubin assays run on the Roche analyzers at DSC, FMC & RRLs.

For patients less than 15 days, Bilirubin testing is not available at BMSH; patients will be redirected to CMGH for collection and testing.

Testing Location Chemistry-All Sites
Rural: CRL
Testing Frequency Daily
Alternate Name(s) Conjugated (Direct) Bilirubin, Total Bilirubin
Reference Interval
Total Bilirubin:

< 1 day

0-100 umol/L

1-2 days

0-150 umol/L

3-14 days

0-220 umol/L

15-365 days

0-23 umol/L

1-4 years

0-10 umol/L

5-9 years

0-14 umol/L

10-14 years

0-19 umol/L

15-150 years

0-24 umol/L

 Direct (Conjugated) Bilirubin:
 < 15 days  0 - 20 umol/L 
 15 days - 150 years   0 - 7 umol/L

Click here for critical values