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CD127 CD132 X-Linked SCID Screen

Source Blood
Mnemonic CD127/CD132
Specimen Requirements

Pediatric: 1 x 1.8 mL blue top sodium citrate

Adult: 1 x 8.5 mL yellow top ACD-A

Minimum volume: 1.0 mL

Also accepted: EDTA, sodium heparin

Specimen Handling

Specimens submitted to CLS from within Alberta: Mix well. DO NOT centrifuge or freeze. Deliver immediately to Flow Cytometry.

Specimens submitted to CLS from outside Alberta: DO NOT centrifuge or freeze samples. Ship using an overnight courier at 4oC.Please follow Flow Cytometry shipping and handling instructions when sending to CLS.

Additional Information

Specimens submitted to CLS from outside Alberta: Use specific Out of Province Requisition Flow Cytometry Out of Province Requisition (FC3201).
For pricing and billing, refer to Flow Cytometry Billing and Pricing.

Flow Cytometry is used to screen for CD127 (IL-7Ra) and CD132 (common gamma chain) for investigation of SCID. The percentage of lymphocytes expressing CD127 and CD132 is reported.

Testing Location Flow Cytometry
Testing Frequency Monday - Saturday
Alternate Name(s) CD127, CD132, common gamma chain, IL-7Ra
Reference Interval Provided on report.