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Calcium - Urine

Source Urine
Mnemonic 24 hour:                           UCAD and U24H
Random:                           UCA
Calcium/Creatinine Ratio:  UCACR
Specimen Requirements Extra-regional sites: see Calcium - Urine, Extra-regional 24 hour and Timed Collection: 
REFRIGERATE during collection.
SUBMIT 5 mL aliquot of acidified 24 hour urine collection.
Specimen may be acidified during or following collection.  See Specimen Handling and Additional Information sections for acidification instructions.|
If test is ordered at the same time as Oxalate, collect with HCl instead of Sulfamic acid. See Oxalate.

Random:
SUBMIT 5 mL aliquot of acidified random urine collection.
Specimen may be acidified during or following collection.  See Specimen Handling and Additional Information sections for acidification instructions.

Calcium/Creatinine Ratio:
5 mL aliquot of acidified 24 hour urine collection preferred; acidified random urine specimen acceptable.
Specimen Handling

Acidify specimen in original collection container.

 ADULT Collection Type 

 Weight of Sulfamic acid in Pouch for
 ADULT Collections

 24 hr. collections on
 patients > 4yrs.

 7 g pouch in each 4 liter container collection
 when acidifying during or after collection

 Adult timed collections

 Use 7 g pouch if >700 mL or when acidifying
 during collection
 0.5 g pouch for every 100 mL collected when
 acidifying after collection up to 700 mL

 Adult random collections

 0.5 g pouch for every 100 mL collected
 (orange capped urine container = 100 mL)
 If less than 100 mL collected: send urine container
 to DSC Chemistry for acidification. DO NOT Aliquot.
 Clearly label - Not Acidified.

Collection Type for     
NEWBORN up to 4 YRS     

Weight of Sulfamic acid Pouch for   
NEWBORN up to 4 YRS

24 hr. collections on
newborn up to 4 yrs.

Use pre-acidification chart in
Additional Information section

Random collections on
newborn up to 4 yrs.

0.5 g pouch for every 100 mL collected.
If less than 100 mL collected: send urine container to
DSC Chemistry for acidification. DO NOT Aliquot.
Clearly label - Not Acidified.

Aliquoting:
If acidified during urine collection:
Mix well and pour off 5 mL of acidified urine to aliquot tube.

If acidified following urine collection:
Add appropriate size of Sulfamic acid pouch, SHAKE bottle vigorously and LET SIT 15 minutes.
Mix and pour off 5 mL of acidified urine to aliquot tube. 

RECORD 24 hour urine volume and collection (start and stop) dates and times in space provided on requisition.

Additional Information

For Pre-Acidification of Pediatric 24 hour Urines:

Age

 Number of 0.5 g pouches of Sulfamic acid
 to add when acidifying during collection

  Up to 1 month   

 Not suitable for 0.5 g pouch
 Send original collection container to DSC Chemistry.
 Clearly label - Not Acidified.

 1 up to 2 months

2

 2 up to 4 months

3

 4 up to 12 months

4

 12 months up to 4 yrs

5
Testing Location Chemistry-DSC
Testing Frequency Daily
Alternate Name(s)
Reference Interval 2.5 - 7.5 mmol/day