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Mitogen Stimulation Assay

Source Blood
Mnemonic MSA
Specimen Requirements

Pediatric: 2 x 4 mL dark green top sodium heparin tubes

Adult: 2 x 4 mL dark green top sodium heparin tubes

Minimum volume: 3.0 mL

Not accepted: EDTA, sodium citrate, ACD-A, lithium heparin

Can only be collected on Wednesdays.

Specimen Handling

Specimens submitted to CLS from within Alberta: Must be collected and delivered on Wednesdays only. Mix well. DO NOT refrigerate, centrifuge or freeze samples.

Specimens submitted to CLS from outside Alberta: Must be delivered on Wednesdays only. DO NOT refrigerate, centrifuge or freeze samples. Ship at room temperature, using an overnight courier. Please follow Flow Cytometry Shipping and Handling instructions when sending to CLS.

Additional Information

Specimens submitted to CLS from outside Alberta: Collect a normal peripheral blood sample and ship with patient sample. Use FC3200EP Flow Cytometry Out of Province Requisition.
For pricing and billing, refer to Flow Cytometry Billing and Pricing.

Peripheral blood lymphocytes are isolated and set up in culture with the T cell mitogens, phytohemagglutinin, concanavalin A, pokeweed mitogen and the B cell mitogen, Staphylococcus A in order to assess proliferative response. The ability of mononuclear cells to proliferate is assessed by flow cytometry cell counts and is reported as a proliferation index. Blast transformation, the percentage of cells showing increased size post-mitogen stimulation, is also reported.

Testing Location Flow Cytometry
Testing Frequency

Thursdays ONLY

Alternate Name(s) Lymphocyte Proliferation, MSA
Reference Interval Provided on report