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Phosphate - Urine

Source Urine
Mnemonic 24 hour:                                 UPHOSD and U24H
Random:                                 UPHOS
Phosphate/Creatinine Ratio:  UPHOSCR (ACH only)
Specimen Requirements

24 hour and Timed Collection:
REFRIGERATE during collection.
Submit 5 mL aliquot of acidified 24 hour urine collection.
Specimen may be acidified during or following collection. See Specimen Handling and Additional Information sections for acidification instructions.
If test is ordered at the same time as Oxalate, collect with HCl instead of Sulfamic acid. See Oxalate.

Random:
Submit 5 mL aliquot of acidified random urine collection.
Specimen may be acidified during or following collection. See Specimen Handling and Additional Information sections for acidification instructions.

Phosphate/Creatinine Ratio:
5 mL aliquot of acidified 24 hour urine collection preferred: acidified random urine specimen acceptable.

Specimen Handling

Acidify specimen in original collection container.

ADULT Collection Type

Weight of Sulfamic acid in pouch for
ADULT Collections

24 hour collections on
patients > 4 yrs.

7 g pouch in each 4 liter container collection when
acidifying during or after collection

Adult timed collections

Use 7 g pouch if > 700 mL or when acidifying
during collection.  0.5 g pouch for every 100 mL
collected when acidifying after collection up to 700 mL
Adult random collections

0.5 g pouch for every 100 mL collected
(orange capped urine container = 100 mL)
If less than 100 mL collected: send urine
container to DSC Chemistry for acidification.
Do Not Aliquot. Clearly label - Not Acidified.

 

Collection Type for
NEWBORN up to 4 YRS

 Weight of Sulfamic acid in pouch for
NEWBORN up to 4 YRS

24 hour collections on
newborn up to 4 years

Use pre-acidification chart in
Additional Information section

Random collections on
newborn up to 4 yrs.

 0.5 g pouch for every 100 mL collected.
If less than 100 mL collected: send urine
container to DSC Chemistry for acidification.
Do Not Aliquot. Clearly label - Not acidified.

Aliquoting:
If acidified during urine collection: 
Mix well and pour off 5 mL of acidified urine to aliquot tube. 

If acidified following urine collection:
Add appropriate size of Sulfamic acid pouch, SHAKE bottle vigorously and LET SIT 15 minutes.
Mix and pour off 5 mL of acidified urine to aliquot tube.

RECORD 24 hour urine volume and collection (start and stop) dates and
times in space provided on requisition.

Additional Information

For Pre-acidification of Pediatric 24 hour Urines:

Age

 Number of 0.5 g pouches to add when
acidifying during collection

Up to 1 month

Not suitable for 0.5 g pouch
Send original collection container to DSC Chemistry.
Clearly label - Not Acidified.

1 up to 2 months

2

2 up to 4 months

3

4 up to 12 months

4

12 months up to 4 yrs.

5

 

Testing Location Chemistry-DSC
Testing Frequency Daily
Alternate Name(s)
Reference Interval 16.0 - 48.0 mmol/d