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Anaerobic Culture, includes aerobic culture - Eye, Critical

Source Eye, Critical
Mnemonic M EYECRITI
Specimen Requirements

Acceptable specimens:
biopsy, corneal scrapings, enucleation, ulcers, keratitis, intraocular fluid, vitreous/aqueous eye fluid, endophthalmitis, lacrimal gland/canal, anterior chamber fluid, washing fluid.

Collect fluid aspirate and inject through the stopper into 
anaerobic transport media (PRAS).

Other specimens may be submitted in a sterile container.

Refer to CLS Form #MI6006:
Critical Eye Specimen Instruction - Guidelines for Physicians.

Specimen Handling

Transport ASAP (priority).
Specimen MUST be received in laboratory within 2 hours  of collection.
Store specimen at room temperature. 
DO NOT refrigerate. 

Specimen may be inoculated to media at the bedside.
Media kits are available from CLS Microbiology.
Transport inoculated media plates and remaining specimen to Microbiology with a gel pack.

Critical eye specimens must be inoculated to media within 1 hour of collection if STAT requested.

 

Additional Information Specify left or right eye on requisition.
Send both inoculated plates and remaining specimen to DSC Microbiology. Turnaround time for negative culture: 4 days.
Testing Location CLS Microbiology
Testing Frequency Daily
Alternate Name(s)  

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